The EXT1 Polyclonal Antibody is a valuable tool for research involving the EXT1 protein, which plays a key role in heparan sulfate biosynthesis. This antibody, developed using rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the EXT1 protein, this antibody enables accurate detection and analysis in various cell types, making it an essential component for studies in glycobiology and extracellular matrix research.
EXT1 is a critical component in the biosynthesis of heparan sulfate, a key molecule involved in cell signaling, development, and homeostasis. Dysregulation of heparan sulfate biosynthesis has been linked to various diseases, including cancer, developmental disorders, and inflammatory conditions. By understanding the function and regulation of EXT1, researchers can uncover new insights into these diseases and potentially develop novel therapeutic interventions targeting heparan sulfate biosynthesis.
Product Name:
EXT1 Polyclonal Antibody
SKU:
CAB24353
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human EXT1(NP_000118.2).
Endoplasmic reticulum membrane, Golgi apparatus membrane, Single-pass type II membrane protein.
Calculated MW:
86kDa
Observed MW:
86kDa
This gene encodes an endoplasmic reticulum-resident type II transmembrane glycosyltransferase involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type I form of multiple exostoses.
Purification Method:
Affinity purification
Gene ID:
2131
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using EXT1 Rabbit pAb (CAB24353) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
