The Exportin-2 Polyclonal Antibody (CAB9510) is a valuable tool for researchers studying Exportin-2, a protein involved in nucleocytoplasmic transport, specifically the export of mRNA from the nucleus to the cytoplasm. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to Exportin-2, this antibody enables the detection and analysis of Exportin-2 protein in a variety of cell types, making it an excellent choice for studies in molecular biology and cellular transport mechanisms.
Exportin-2 plays a crucial role in the regulation of gene expression by facilitating the transport of mRNA molecules out of the nucleus, where they can be translated into proteins. Dysregulation of Exportin-2 activity has been implicated in various diseases, including cancer and neurological disorders, highlighting the importance of further research into this protein. Understanding the function of Exportin-2 is essential for advancing our knowledge of cellular mechanisms and developing potential therapeutic strategies for diseases associated with aberrant gene expression.
Product Name:
Exportin 2 (XPO2) Rabbit Polyclonal Antibody
SKU:
CAB9510
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Exportin 2 (XPO2) (NP_001243064.1).
Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation. Alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1434
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Exportin 2 (XPO2) antibody (CAB9510) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
