The Exostosin-like 3 Polyclonal Antibody (CAB3857) is a valuable tool for research involving Exostosin-like 3 (EXTL3), a glycosyltransferase enzyme involved in the biosynthesis of heparan sulfate. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the EXTL3 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in glycobiology and cancer research.EXTL3 plays a critical role in the modification of heparan sulfate chains, which are essential components of the extracellular matrix and play key roles in cell signaling and adhesion.
Dysregulation of EXTL3 has been implicated in various diseases, including cancer and developmental disorders. Therefore, understanding the function of EXTL3 and its role in heparan sulfate biosynthesis is crucial for advancing research in these areas and developing targeted therapies.Overall, the Exostosin-like 3 Polyclonal Antibody is a valuable tool for researchers studying the intricate mechanisms of heparan sulfate biosynthesis and its implications in health and disease. Its high specificity and reactivity make it a reliable choice for investigating the functions of EXTL3 in various biological contexts.
Product Name:
EXTL3 Rabbit Polyclonal Antibody
SKU:
CAB3857
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 52-220 of human EXTL3 (NP_001431.1).
Endoplasmic reticulum membrane, Single-pass type II membrane protein.
Calculated MW:
105kDa
Observed MW:
105kDa
This gene encodes a single-pass membrane protein which functions as a glycosyltransferase. The encoded protein catalyzes the transfer of N-acetylglucosamine to glycosaminoglycan chains. This reaction is important in heparin and heparan sulfate synthesis. Alternative splicing results in the multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2137
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using EXTL3 Rabbit pAb (CAB3857) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
