The EWSR1 Rabbit Monoclonal Antibody (CAB9640) is specially designed for research involving EWSR1, a fusion protein implicated in various cancer types, especially Ewing's sarcoma. This antibody, derived from rabbits, exhibits high reactivity with human samples and is validated for use in immunohistochemistry and Western blot applications. By binding specifically to the EWSR1 protein, this antibody facilitates the detection and analysis of EWSR1 expression in different cell types, making it an ideal tool for studies in oncology and cancer biology.EWSR1, a key player in various oncogenic processes, is frequently involved in chromosomal translocations leading to the formation of oncogenic fusion proteins.
Understanding the role of EWSR1 in cancer development and progression is crucial for developing targeted therapies and diagnostic tools for Ewing's sarcoma and other cancers with EWSR1 rearrangements. The EWSR1 Rabbit Monoclonal Antibody provides researchers with a reliable tool to study the expression and function of EWSR1 in cancer cells, ultimately contributing to advancements in cancer research and personalized medicine.
Product Name:
EWSR1 Rabbit Monoclonal Antibody
SKU:
CAB9640
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human EWSR1 (Q01844).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
EWS; EWS-FLI1; bK984G1.4; EWSR1
Positive Sample:
HeLa,Jurkat,Mouse testis,Mouse thymus,Rat testis
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm, Nucleus.
Calculated MW:
68kDa
Observed MW:
90kDa
This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.
Purification Method:
Affinity purification
Gene ID:
2130
Clone Number:
ARC1674
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using EWSR1 Rabbit mAb (CAB9640) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
