The ETV2 Polyclonal Antibody (PAC06275) is a valuable tool for researchers studying the ETV2 transcription factor, which plays a crucial role in endothelial cell development and angiogenesis. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in various applications, including Western blot and immunohistochemistry.ETV2 is known for its ability to promote the formation of blood vessels and regulate endothelial cell differentiation, making it a key player in cardiovascular development and disease.
Research into ETV2 has implications for understanding vascular development, tumor angiogenesis, and cardiovascular disorders. By targeting ETV2 with this antibody, researchers can gain valuable insights into the mechanisms underlying these processes and potentially identify new therapeutic targets for conditions related to angiogenesis and blood vessel formation.
Antibody Name:
ETV2 Antibody (PACO62275)
Antibody SKU:
PACO62275
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human ETS translocation variant 2 protein (74-201AA)
Immunofluorescence staining of HepG2 cells with PACO62275 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Binds to DNA sequences containing the consensus pentanucleotide 5'-CGGA[AT]-3'.
Synonyms:
ETS translocation variant 2 (Ets-related protein 71), ETV2, ER71 ETSRP71
UniProt Protein Function:
ETV2: Binds to DNA sequences containing the consensus pentanucleotide 5'-CGGA[AT]-3'. Belongs to the ETS family.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 19q13.12Cellular Component: nucleusBiological Process: cell differentiation; regulation of transcription from RNA polymerase II promoter
