The ETV1 Polyclonal Antibody (CAB7184) is a valuable tool for researchers studying ETV1, a transcription factor involved in various cellular processes, including cell differentiation and proliferation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the ETV1 protein, this antibody enables the detection and analysis of ETV1 expression in a variety of cell types, making it an ideal choice for studies in cancer research and developmental biology.
ETV1 is known to play a crucial role in oncogenesis, particularly in certain types of tumors where it is overexpressed or mutated. Understanding the function and regulation of ETV1 is essential for unraveling its contribution to cancer development and progression. This antibody provides researchers with a reliable tool to explore the role of ETV1 in cancer biology, potentially leading to new insights and therapeutic strategies in the treatment of ETV1-related malignancies.
Product Name:
ETV1 Rabbit Polyclonal Antibody
SKU:
CAB7184
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ETV1 (NP_004947.2).
This gene encodes a member of the ETS (E twenty-six) family of transcription factors. The ETS proteins regulate many target genes that modulate biological processes like cell growth, angiogenesis, migration, proliferation and differentiation. All ETS proteins contain an ETS DNA-binding domain that binds to DNA sequences containing the consensus 5'-CGGA[AT]-3'. The protein encoded by this gene contains a conserved short acidic transactivation domain (TAD) in the N-terminal region, in addition to the ETS DNA-binding domain in the C-terminal region. This gene is involved in chromosomal translocations, which result in multiple fusion proteins including EWS-ETV1 in Ewing sarcoma and at least 10 ETV1 partners (see PMID: 19657377, Table 1) in prostate cancer. In addition to chromosomal rearrangement, this gene is overexpressed in prostate cancer, melanoma and gastrointestinal stromal tumor. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
2115
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ETV1 antibody (CAB7184) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
