The Ethylene Insensitive 3 Polyclonal Antibody (CAB18629) is a powerful tool for researchers studying the ethylene signaling pathway in plants. This antibody, generated in rabbits, specifically targets the Ethylene Insensitive 3 protein, a key component in ethylene signal transduction.With its high reactivity towards plant samples, this antibody is ideal for use in Western blot applications, allowing for the precise detection and analysis of Ethylene Insensitive 3 protein levels in various plant tissues and cell types. This makes it a valuable asset for researchers interested in unraveling the complexities of ethylene signaling and its role in plant growth and development processes.
By understanding the function of Ethylene Insensitive 3, researchers can gain insights into how plants respond to environmental cues and stressors, ultimately leading to advancements in crop improvement strategies and plant biotechnology. Whether studying plant physiology, signaling pathways, or stress responses, the Ethylene Insensitive 3 Polyclonal Antibody is an essential tool for researchers looking to broaden their understanding of plant biology.
Product Name:
OsEIL1 Rabbit Polyclonal Antibody
SKU:
CAB18629
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Oryza sativa
Immunogen:
LSRGKDPAGGVVGDPSK-C
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
EIL1; MHZ6; EIL1A; OsEIL1; OsEIL1a; OsJ_10672
Positive Sample:
Leaves
Conjugate:
Unconjugated
Cellular Localization:
nucleus
Calculated MW:
71kDa
Observed MW:
90kDa
Transcription factor acting as a positive regulator in the ethylene response pathway). Required for the inhibition of root growth by ethylene in etiolated seedlings. Functions upstream of the auxin biosynthetic gene YUCCA8 and directly activates its expression . Functions downstream of the ethylene signaling factor EIN2 in disease resistance against the rice blast fungus (Magnaporthe oryzae. Binds directly to the promoters of the NADPH oxidases RBOHA and RBOHB, and the jasmonate biosynthetic gene OPR4 to activate their expression during fungal infection. May enhance disease resistance by facilitating reactive oxygen species (ROS) generation and jasmonate biosynthesis with subsequent phytoalexin accumulation during Magnaporthe oryzae infection. Acts as negative regulator of salt tolerance . During salt stress, activates the cation transporter HKT1, which mediates increased sodium uptake in roots, and contributes to sodium accumulation and salt toxicity. Binds directly to the DNA sequence 5'-TGTTACAAATACC-3' in the promoter of the GA20OX2 gene to activate its expression at the transcriptional level during ethylene signaling. Possesses transactivation activity in protoplasts.
Purification Method:
Affinity purification
Gene ID:
107276358
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsEIL1 Rabbit pAb (CAB18629) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
