The ETHE1 Polyclonal Antibody (CAB10142) is a vital tool for researchers studying the ETHE1 protein, a key enzyme involved in sulfur metabolism and mitochondrial function. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in various applications, including Western blot and immunohistochemistry.The ETHE1 protein plays a crucial role in maintaining cellular homeostasis by catalyzing the conversion of persulfide to sulfide, a process essential for proper mitochondrial function and overall cellular health. Dysregulation of ETHE1 has been implicated in a variety of metabolic disorders and mitochondrial diseases, making it a promising target for therapeutic interventions.
By utilizing the ETHE1 Polyclonal Antibody, researchers can accurately detect and analyze ETHE1 protein levels in different cell types and tissues, allowing for deeper insight into its function and potential implications in disease pathology. This antibody is an invaluable tool for studies in biochemistry, metabolism, and mitochondrial physiology, offering new avenues for exploring the intricacies of sulfur metabolism and its impact on human health.
Product Name:
ETHE1 Rabbit Polyclonal Antibody
SKU:
CAB10142
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 8-245 of human ETHE1 (NP_055112.2).
HT-29,HepG2,Mouse large intestine,Mouse liver,Rat liver
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Mitochondrion matrix, Nucleus.
Calculated MW:
28kDa
Observed MW:
28kDa
This gene encodes a member of the metallo beta-lactamase family of iron-containing proteins involved in the mitochondrial sulfide oxidation pathway. The encoded protein catalyzes the oxidation of a persulfide substrate to sulfite. Certain mutations in this gene cause ethylmalonic encephalopathy, an infantile metabolic disorder affecting the brain, gastrointestinal tract and peripheral vessels. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
23474
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ETHE1 Rabbit pAb (CAB10142) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
