Ethanol Test Kit (BA0039)

SKU:

BA0039

Product Type:

Assay

Instrument:

Microplate Reader

Sample Type:

Serum, Plasma, Urine, Alcoholic Beverages

Research Area:

Oxidative Stress

Reactive Oxygen Species

Plant & Environmental Stress

Clinical Chemistry

Food Safety & Analysis

Description

Description

Ethanol Test Kit - Information

This colorimetric ethanol test kit is based on an improved dichromate method, in which dichromate is reduced by ethanol to a bluish chromic (Cr³⁺) product. The intensity of color, measured at 580 nm, is a direct measure of the alcohol concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits high sensitivity.

Applications

For quantitative determination of ethanol and alcohol metabolism.

Ethanol Test Kit - Key Features

Sensitive and accurate. Detection range 0.04 - 4% alcohol in 96-well plate assay.
Convenient and high-throughput. The procedure involves adding a single working reagent, incubation for 8 min, adding a Stop Reagent, and reading the optical density. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Versatility. Assays can be executed in 96-well plate or cuvet.

Ethanol Test Kit - Data Sheet
Kit Includes	Reagent A: 50 mL Reagent B: 50 mL 10% TCA: 50 mL Standard: 2 mL 10% (v/v) ethanol
Kit Requires	Pipeting (multi-channel) devices.
Method of Detection	OD580nm (Chemical)
Detection Limit	0.04%
Samples	Alcoholic beverages etc
Species	All
Protocol Length	10 min
Size	500 tests
Storage	Store reagents at room temperature and the ethanol standard at 4°C.
Shelf Life	12 months

More Details

Alcoholic drinksare among the daily consumed beverages. Studies have shown heavy alcohol consumption may lead to various forms of liver diseases and to increased mortality rates. Quantitative determination of alcohol (ethanol, C₂H₅OH) finds applications in basic research, drug discovery, clinic studies and winery. Simple, direct and automation-ready procedures for measuring ethanol concentration are very desirable.