The ETFA Polyclonal Antibody (CAB7670) is a highly specific research tool designed for studying the ETFA protein, an essential component of the electron transfer flavoprotein complex involved in fatty acid beta-oxidation. This antibody, generated in rabbits, demonstrates strong reactivity with human samples and has been validated for use in Western blot applications.ETFA plays a crucial role in cellular energy metabolism and dysfunction in this protein has been linked to disorders like multiple acyl-CoA dehydrogenase deficiency. By targeting ETFA, researchers can gain insights into metabolic pathways and their implications in various diseases, making this antibody a valuable tool for studies in biochemistry, metabolism, and mitochondrial disorders.
With its ability to detect ETFA in various cell types, the CAB7670 Polyclonal Antibody is an ideal choice for scientists conducting research in areas such as metabolism, mitochondrial function, and rare genetic diseases. By understanding the role of ETFA in cellular processes, researchers can advance knowledge and potentially develop targeted therapies for metabolic disorders.
Product Name:
ETFA Rabbit Polyclonal Antibody
SKU:
CAB7670
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-333 of human ETFA (NP_000117.1).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
EMA; GA2; MADD; ETFA
Positive Sample:
Mouse heart,Rat heart,Mouse liver,Rat liver
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion matrix.
Calculated MW:
35kDa
Observed MW:
35kDa
ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2108
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using ETFA Rabbit pAb (CAB7670) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
