The ETF1 Polyclonal Antibody (CAB5920) is a valuable tool for researchers studying ETF1, a protein involved in translation termination and peptide release. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the ETF1 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential reagent in the fields of molecular biology and protein research.
ETF1 is a key player in the final stages of protein synthesis, ensuring accurate termination of translation and release of the newly synthesized peptide. Dysregulation of ETF1 has been implicated in various diseases, making it an attractive target for studies on genetic disorders, neurodegenerative diseases, and cancer. Understanding the function of ETF1 is essential for unraveling the intricacies of protein synthesis and identifying potential therapeutic targets for disease intervention.
Product Name:
ETF1 Rabbit Polyclonal Antibody
SKU:
CAB5920
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 278-437 of human ETF1 (NP_004721.1).
This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.
Purification Method:
Affinity purification
Gene ID:
2107
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ETF1 antibody (CAB5920) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
