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ESRα Rabbit Polyclonal Antibody (CAB0296)

SKU:
CAB0296
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
€139 - €419
Frequently bought together:

Description

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Product Name:ESRα Rabbit Polyclonal Antibody
SKU:CAB0296
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence within amino acids 516-595 of human ESRα (NP_000116.2).
Sequence:HMSN KGME HLYS MKCK NVVP LYDL LLEM LDAH RLHA PTSR GGAS VEET DQSH LATA GSTS SHSL QKYY ITGE AEGF PATV
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:ER; ESR; Era; ESRA; ESTRR; NR3A1; ESRα
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic side, Golgi apparatus, Nucleus, Peripheral membrane protein, Single-pass type I membrane protein.
Calculated MW:66kDa
Observed MW:66kDa

This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain.

Purification Method:Affinity purification
Gene ID:2099
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of HeLa cells, using ESRα antibody (CAB0296) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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