The ESR1 Monoclonal Antibody (CAB12976) is a valuable tool for researchers studying the estrogen receptor alpha (ESR1), a nuclear receptor protein that plays a key role in the regulation of gene expression in response to estrogen. This antibody, produced in mouse, is highly specific for human samples and has been validated for use in various immunological assays, including Western blotting and immunohistochemistry.ESR1 is a crucial biomarker in hormone-related cancers, such as breast cancer, where it is often overexpressed and drives tumor growth. By targeting ESR1 with this monoclonal antibody, researchers can better understand the mechanisms underlying hormone signaling pathways and potentially identify new therapeutic targets for cancer treatment.
Furthermore, this antibody can help researchers investigate the role of ESR1 in other physiological processes, such as reproductive health and bone metabolism. Its high sensitivity and specificity make it a reliable tool for exploring the diverse functions of ESR1 in both normal and disease states, offering insights that could lead to new advancements in personalized medicine and targeted therapies.
Product Name:
ESRα Rabbit Monoclonal Antibody
SKU:
CAB12976
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Estrogen Receptor alpha (ESRα) (P03372).
Cell membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic side, Golgi apparatus, Nucleus, Peripheral membrane protein, Single-pass type I membrane protein.
Calculated MW:
66kDa
Observed MW:
66kDa
This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain.
Purification Method:
Affinity purification
Gene ID:
2099
Clone Number:
ARC0199
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from MCF7 cells, using ESRα Rabbit mAb (CAB12976) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
