The Estrogen Receptor Polyclonal Antibody (CAB0296) is a valuable tool for researchers studying estrogen receptor, a key regulator of gene expression and cellular growth. This antibody, produced in rabbits, is highly specific for detecting estrogen receptor in human samples and is validated for use in Western blot applications. By binding to the estrogen receptor protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for studies in endocrinology, cancer research, and reproductive biology.The estrogen receptor plays a crucial role in mediating the effects of estrogen on target tissues, influencing a wide range of physiological processes such as cell proliferation, differentiation, and survival.
Dysregulation of estrogen receptor signaling has been implicated in the development and progression of various diseases, including breast cancer and osteoporosis. Therefore, understanding the expression and activity of estrogen receptor is essential for advancing our knowledge of hormone-related disorders and developing targeted therapies.Overall, the Estrogen Receptor Polyclonal Antibody (CAB0296) provides researchers with a reliable tool for investigating the role of estrogen receptor in health and disease, paving the way for new discoveries and advancements in the field of molecular biology.
Product Name:
ESRα Rabbit Polyclonal Antibody
SKU:
CAB0296
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence within amino acids 516-595 of human ESRα (NP_000116.2).
Cell membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic side, Golgi apparatus, Nucleus, Peripheral membrane protein, Single-pass type I membrane protein.
Calculated MW:
66kDa
Observed MW:
66kDa
This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain.
Purification Method:
Affinity purification
Gene ID:
2099
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using ESRα antibody (CAB0296) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
