The ESPL1 Polyclonal Antibody (CAB15366) is a valuable tool for researchers studying the ESPL1 protein, also known as Separase, an essential regulator of cell division. This antibody, produced in rabbits, is highly specific to human ESPL1 and is validated for use in Western blot applications. By binding to the ESPL1 protein, this antibody allows for the precise detection and analysis of ESPL1 expression in various cell types, making it an ideal choice for studies in cell biology and cancer research.ESPL1 plays a crucial role in the accurate segregation of chromosomes during cell division, making it a key player in maintaining genomic stability.
Dysregulation of ESPL1 has been implicated in various cancers and genetic disorders, highlighting its importance as a therapeutic target. Understanding the function of ESPL1 is essential for unraveling the mechanisms underlying these diseases and developing targeted treatments to combat them.Overall, the ESPL1 Polyclonal Antibody offers researchers a reliable tool for investigating the role of ESPL1 in cell division and disease, providing valuable insights into potential therapeutic strategies for cancer and genetic disorders.
Product Name:
ESPL1 Rabbit Polyclonal Antibody
SKU:
CAB15366
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1220-1500 of human ESPL1 (NP_036423.4).
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).
Purification Method:
Affinity purification
Gene ID:
9700
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ESPL1 Rabbit pAb (CAB15366) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
