The ESAM Polyclonal Antibody (CAB12210) is specifically designed for research involving Endothelial Cell-Selective Adhesion Molecule (ESAM), a cell adhesion molecule known to play a critical role in various biological processes, including leukocyte recruitment and inflammation. This antibody is produced in rabbits and has been validated for use in Western blot applications, allowing for the detection and analysis of ESAM protein in human samples. Its high reactivity and specificity make it an ideal tool for studying ESAM expression and function in different cell types, particularly in the fields of vascular biology and immune response regulation.
ESAM has been implicated in various physiological and pathological conditions, making it a valuable target for research in areas such as cardiovascular diseases, inflammatory disorders, and cancer. By understanding the role of ESAM in these contexts, researchers can gain insights into potential therapeutic strategies aimed at modulating its activity for therapeutic intervention.
Product Name:
ESAM Rabbit Polyclonal Antibody
SKU:
CAB12210
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-248 of human ESAM (NP_620411.2).
Cell junction, Cell membrane, Single-pass type I membrane protein, adherens junction, tight junction.
Calculated MW:
41kDa
Observed MW:
60kDa
Enables cell-cell adhesion mediator activity. Involved in several processes, including bicellular tight junction assembly; cell-cell adhesion; and regulation of actin cytoskeleton organization. Located in cell-cell junction and plasma membrane. Part of protein-containing complex.
Purification Method:
Affinity purification
Gene ID:
90952
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ESAM Rabbit pAb (CAB12210) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
