The ERVW-1 Polyclonal Antibody (CAB16522) is specifically designed for research involving ERVW-1, a protein associated with fertility and embryo implantation. This antibody, produced in rabbits, is highly reactive with human samples and is validated for use in applications such as Western blotting. By binding to the ERVW-1 protein, this antibody allows for the detection and analysis of ERVW-1 in various types of cells, making it well-suited for studies in reproductive biology and infertility research.ERVW-1, also known as syncytin-1, plays a crucial role in the fusion of placental cells and is essential for the development of a healthy pregnancy.
Dysregulation of ERVW-1 has been linked to pregnancy complications and infertility, making it a key target for investigation in reproductive medicine. Understanding the function of ERVW-1 is vital for developing diagnostic tools and potential therapeutic interventions for individuals experiencing difficulties with fertility.In summary, the ERVW-1 Polyclonal Antibody (CAB16522) offers researchers a valuable tool for studying the role of ERVW-1 in reproduction and fertility. Its high reactivity and specificity make it a reliable choice for experiments aimed at unraveling the complexities of embryo implantation and placental development.
Antibody Name:
Anti-ERVW-1 Antibody
Antibody SKU:
CAB16522
Antibody Size:
20uL, 50uL, 100uL
Application:
WB
Reactivity:
Human, Mouse
Host Species:
Rabbit
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ERVW-1 (NP_001124397.1).
Application:
WB
Recommended Dilution:
WB 1:500 - 1:2000
Reactivity:
Human, Mouse
Positive Samples:
U-251MG, 293T, mouse brain
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ERVW-1 (NP_001124397.1).
Purification Method:
Affinity purification
Storage Buffer:
Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Many different human endogenous retrovirus (HERV) families are expressed in normal placental tissue at high levels, suggesting that HERVs are functionally important in reproduction. This gene is part of an HERV provirus on chromosome 7 that has inactivating mutations in the gag and pol genes. This gene is the envelope glycoprotein gene which appears to have been selectively preserved. The gene's protein product is expressed in the placental syncytiotrophoblast and is involved in fusion of the cytotrophoblast cells to form the syncytial layer of the placenta. The protein has the characteristics of a typical retroviral envelope protein, including a furin cleavage site that separates the surface (SU) and transmembrane (TM) proteins which form a heterodimer. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
UniProt Protein Function:
ERVWE1: Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has retained its original fusogenic properties and participates in trophoblast fusion during placenta morphogenesis. Belongs to the gamma type-C retroviral envelope protein family. HERV class-I W env subfamily.
UniProt Protein Details:
Protein type:Membrane protein, integral; Cell surface
Many different human endogenous retrovirus (HERV) families are expressed in normal placental tissue at high levels, suggesting that HERVs are functionally important in reproduction. This gene is part of an HERV provirus on chromosome 7 that has inactivating mutations in the gag and pol genes. This gene is the envelope glycoprotein gene which appears to have been selectively preserved. The gene's protein product is expressed in the placental syncytiotrophoblast and is involved in fusion of the cytotrophoblast cells to form the syncytial layer of the placenta. The protein has the characteristics of a typical retroviral envelope protein, including a furin cleavage site that separates the surface (SU) and transmembrane (TM) proteins which form a heterodimer. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]
ENV; ENVW; HERVW; ERVWE1; HERV7Q; HERV-7q; HERVWENV; HERV-W-ENVÂ Â
NCBI Protein Information:
syncytin-1
UniProt Protein Name:
Syncytin-1
UniProt Synonym Protein Names:
Endogenous retrovirus group W member 1; Env-W; Envelope polyprotein gPr73; Enverin; HERV-7q Envelope protein; HERV-W envelope protein; HERV-W_7q21.2 provirus ancestral Env polyprotein; Syncytin
Protein Family:
Syncytin
UniProt Gene Name:
ERVW-1Â Â
UniProt Entry Name:
SYCY1_HUMAN
Western blot analysis of extracts of various cell lines, using ERVW-1 antibody (CAB16522) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
