The ERRFI1 Polyclonal Antibody (CAB13099) is a valuable tool for researchers studying ERRFI1, a protein involved in the regulation of cell growth and survival pathways. This antibody, generated in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blotting applications. By binding to ERRFI1, this antibody enables the detection and analysis of ERRFI1 expression in a variety of cell types, making it an excellent choice for studies in cancer biology and molecular biology.ERRFI1, also known as MIG-6 (mitogen-inducible gene 6), acts as a negative regulator of growth factor signaling pathways, such as the epidermal growth factor receptor (EGFR) pathway.
Its role in inhibiting cell growth and promoting apoptosis highlights its importance in controlling cellular processes that contribute to tumorigenesis. Research into ERRFI1 is crucial for understanding its potential as a therapeutic target in cancer treatment and for developing strategies to overcome resistance to targeted therapies. By studying ERRFI1, researchers can uncover new insights into the mechanisms of cancer progression and identify novel approaches for therapeutic intervention.
Product Name:
ERRFI1 Rabbit Polyclonal Antibody
SKU:
CAB13099
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 223-462 of human ERRFI1 (NP_061821.1).
ERRFI1 is a cytoplasmic protein whose expression is upregulated with cell growth (Wick et al., 1995 [PubMed 7641805]). It shares significant homology with the protein product of rat gene-33, which is induced during cell stress and mediates cell signaling (Makkinje et al., 2000 [PubMed 10749885]; Fiorentino et al., 2000 [PubMed 11003669]).
Purification Method:
Affinity purification
Gene ID:
54206
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from rat lung, using ERRFI1 Rabbit pAb (CAB13099) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 5min.
