The ERP44 Polyclonal Antibody (CAB4526) is a valuable tool for researchers studying ER protein 44 (ERP44), a crucial player in protein folding and quality control within the endoplasmic reticulum. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the ERP44 protein, it enables accurate detection and analysis in a variety of cell types, making it ideal for studies in cell biology, protein folding, and disease mechanisms.
ERP44 is a critical component of the ER quality control system, ensuring that proteins are correctly folded and preventing the accumulation of misfolded proteins that can be toxic to cells. Dysregulation of ERP44 function has been implicated in a range of diseases, including neurodegenerative disorders, diabetes, and cancer. The ability to study ERP44 with precision and sensitivity using this antibody provides researchers with valuable insights into the mechanisms of these diseases and potential therapeutic targets.
Product Name:
ERP44 Rabbit Polyclonal Antibody
SKU:
CAB4526
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 57-406 of human ERP44 (NP_055866.1).
This gene encodes a member of the protein disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins. It has an inferred N-terminal signal peptide, a catalytically active thioredoxin (TRX) domain, two TRX-like domains and a C-terminal ER-retention sequence. This protein functions as a pH-regulated chaperone of the secretory pathway and likely plays a role in protein quality control at the endoplasmic reticulum - Golgi interface.
Purification Method:
Affinity purification
Gene ID:
23071
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ERP44 Rabbit pAb (CAB4526) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
