The ERP27 Polyclonal Antibody (CAB15944) is a valuable tool for researchers studying ERP27, a protein involved in endoplasmic reticulum (ER) quality control and protein folding. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications. ERP27, an ER-resident protein, plays a crucial role in maintaining ER function and homeostasis. By binding to ERP27, this antibody allows for the detection and analysis of ERP27 levels in various cell types, facilitating research in fields such as cell biology and protein quality control mechanisms.
The role of ERP27 in protein folding and ER stress response makes it a potential target for studies related to neurodegenerative diseases, cancer, and diabetes, where protein misfolding and ER dysfunction play a critical role. Understanding the function and regulation of ERP27 can provide insights into potential therapeutic strategies for these conditions.
Product Name:
ERP27 Rabbit Polyclonal Antibody
SKU:
CAB15944
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human ERP27 (NP_689534.1).
This gene encodes a noncatalytic member of the protein disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins. The canonical protein has an N-terminal signal sequence, two thioredoxin (TRX)-like domains and a C-terminal ER-retention sequence. Alternative splicing results in multiple transcript variants encoding distinct isoforms; some of which lack domains present in the canonical protein.
Purification Method:
Affinity purification
Gene ID:
121506
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ERP27 Rabbit pAb (CAB15944) at 1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
