The ERN2 Polyclonal Antibody (PAC019618) is a valuable tool for researchers studying ERN2, a protein involved in the unfolded protein response (UPR) pathway. The antibody, generated in rabbits, exhibits high specificity and sensitivity in detecting ERN2 in human samples, making it suitable for use in Western blot and immunohistochemistry applications. By targeting ERN2, this antibody enables researchers to investigate the role of ERN2 in UPR signaling and its implications in diseases such as cancer, neurodegenerative disorders, and metabolic conditions.
ERN2, also known as endoplasmic reticulum to nucleus signaling 2, is a key player in the UPR pathway, which is activated in response to cellular stress and misfolded proteins. Dysregulation of the UPR pathway, including ERN2, has been implicated in various diseases, making ERN2 a potential therapeutic target. The ERN2 Polyclonal Antibody provides researchers with a valuable tool to further explore the function of ERN2 and its involvement in disease pathogenesis, ultimately contributing to the development of targeted therapies.
Antibody Name:
ERN2 Antibody (PACO19618)
Antibody SKU:
PACO19618
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:50-1:200
Species Reactivity:
Human
Immunogen:
Synthetic peptide of human ERN2
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using PACO19618(ERN2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO19618(ERN2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
The accumulation of unfolded proteins within the endoplasmic recticulum (ER) of yeast and mammalian cells activates the unfolded protein response (UPR) pathway and leads to the transcription of ER-specific genes involved in protein folding. The activation of the UPR requires the ER transmembrane kinase IRE1p (for inositol-requiring and ER-to-nucleus signaling protein). IRE1 α and IRE1 β are two mammalian homologs of the yeast IRE1p. These related proteins localize to the ER lumen and contain both a short transmembrane domain that spans the ER membrane and a cytosolic Ser/Thr kinase domain. IRE1 activation involves the oligomerization and trans-phosphorylation of the cytosolic portion of the proteins, which then potentiates its intrinsic kinase activity and, in turn, stimulates transcription of UPR-targeted genes.
Synonyms:
endoplasmic reticulum to nucleus signaling 2
UniProt Protein Function:
IRE2: Induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Apoptosis; Protein kinase, Other; Membrane protein, integral; Ribonuclease; Other group; IRE familyChromosomal Location of Human Ortholog: 16p12.2Molecular Function: ATP binding; endonuclease activity; magnesium ion binding; protein serine/threonine kinase activityBiological Process: apoptotic chromosome condensation; negative regulation of transcription, DNA-dependent; protein amino acid phosphorylation; rRNA catabolic process
