The ERN2 Antibody (PAC019617) is a highly specific and sensitive tool for researchers studying ERN2, a protein involved in endoplasmic reticulum stress response. This polyclonal antibody, produced in rabbits, is optimized for detection in human samples and has been validated for use in Western blotting and immunofluorescence applications. By binding to ERN2, this antibody enables precise detection and analysis of this key protein in various cell types, making it an ideal tool for investigating the role of ERN2 in cellular stress response pathways.ERN2, also known as Endoplasmic Reticulum to Nucleus Signaling 2, plays a critical role in maintaining cellular homeostasis by responding to endoplasmic reticulum stress and initiating signaling pathways to promote cell survival or apoptosis.
Dysregulation of ERN2 has been implicated in various diseases such as neurodegenerative disorders, diabetes, and cardiovascular diseases. Understanding the function of ERN2 is essential for elucidating its role in disease pathology and developing targeted treatments to modulate ER stress responses.Overall, the ERN2 Antibody (PAC019617) is a valuable tool for researchers studying cellular stress responses and investigating the role of ERN2 in various physiological and pathological conditions. Its high specificity and sensitivity make it a reliable choice for experiments in molecular biology, cell biology, and disease research.
Antibody Name:
ERN2 Antibody (PACO19617)
Antibody SKU:
PACO19617
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:50-1:200
Species Reactivity:
Human
Immunogen:
Synthetic peptide of human ERN2
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO19617(ERN2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO19617(ERN2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
The accumulation of unfolded proteins within the endoplasmic recticulum (ER) of yeast and mammalian cells activates the unfolded protein response (UPR) pathway and leads to the transcription of ER-specific genes involved in protein folding. The activation of the UPR requires the ER transmembrane kinase IRE1p (for inositol-requiring and ER-to-nucleus signaling protein). IRE1 α and IRE1 β are two mammalian homologs of the yeast IRE1p. These related proteins localize to the ER lumen and contain both a short transmembrane domain that spans the ER membrane and a cytosolic Ser/Thr kinase domain. IRE1 activation involves the oligomerization and trans-phosphorylation of the cytosolic portion of the proteins, which then potentiates its intrinsic kinase activity and, in turn, stimulates transcription of UPR-targeted genes.
Synonyms:
endoplasmic reticulum to nucleus signaling 2
UniProt Protein Function:
IRE2: Induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Apoptosis; Protein kinase, Other; Membrane protein, integral; Ribonuclease; Other group; IRE familyChromosomal Location of Human Ortholog: 16p12.2Molecular Function: ATP binding; endonuclease activity; magnesium ion binding; protein serine/threonine kinase activityBiological Process: apoptotic chromosome condensation; negative regulation of transcription, DNA-dependent; protein amino acid phosphorylation; rRNA catabolic process
