The ERN1 Polyclonal Antibody (AB10001) is a valuable tool for researchers studying ERN1, a critical protein involved in the unfolded protein response (UPR) pathway. This rabbit polyclonal antibody is highly specific to ERN1 and has been validated for use in various applications, including Western blot, immunohistochemistry, and immunofluorescence.ERN1, also known as IRE1α, is a key sensor of endoplasmic reticulum (ER) stress and plays a crucial role in initiating the UPR to restore ER homeostasis. Dysregulation of the UPR pathway has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic diseases.
Therefore, studying ERN1 and its downstream signaling pathways is essential for understanding the mechanisms underlying these conditions and developing targeted therapies.With its high specificity and sensitivity, the ERN1 Polyclonal Antibody is a valuable tool for researchers investigating the role of ERN1 in cellular stress responses and disease pathogenesis. Its ability to detect ERN1 in various cell types and tissues makes it an ideal choice for studies in molecular biology, cell biology, and biomedical research.
Product Name:
IRE1 Rabbit Polyclonal Antibody
SKU:
CAB17940
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 286-435 of human IRE1 (NP_001424.3).
Endoplasmic reticulum membrane, Single-pass type I membrane protein.
Calculated MW:
110kDa
Observed MW:
130kDa
This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.
Purification Method:
Affinity purification
Gene ID:
2081
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using IRE1 Rabbit pAb (CAB17940) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 120s.
