The ERI1 Polyclonal Antibody (PACO56386) is a powerful tool for researchers studying ERI1, a key player in RNA metabolism and post-transcriptional regulation. This antibody, generated in rabbits, demonstrates high specificity and sensitivity towards ERI1 in human samples, making it reliable for Western blot applications.ERI1, also known as exoribonuclease 1, is involved in RNA degradation and turnover processes, impacting gene expression and cellular function. Its role in maintaining RNA stability and regulating RNA quality control makes it a crucial component in various biological pathways, including mRNA decay and microRNA regulation.
By targeting ERI1 with this antibody, researchers can accurately detect and analyze the protein in different cell types, allowing for detailed investigations into its functions and interactions. This makes the ERI1 Polyclonal Antibody an essential tool for studies in RNA biology, gene regulation, and molecular mechanisms underlying diseases such as cancer and neurodegenerative disorders. Gain valuable insights into ERI1-mediated processes with this reliable research reagent.
Western Blot. Positive WB detected in: Jurkat whole cell lysate. All lanes: ERI1 antibody at 7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 41 kDa. Observed band size: 46 kDa.
IHC image of PACO56386 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PACO56386 diluted at 1:400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.
THEX1: RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication- dependent histone pre-mRNAs. Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1. Interacts in a cooperative manner with SLBP to the mature 3'-end of histone mRNAs. Binds to 40S and 60S ribosomal subunits and to 80S assembled ribosomes. Found in a ternary complex with SLBP and the stem-loop structure of the 3'-end of histone mRNAs. Although it can bind simultaneously with SLBP to the 3'-end of histone mRNA, the presence of SLBP prevents the exonuclease activity.Protein type: EC 3.1.-.-; Hydrolase; NucleolusChromosomal Location of Human Ortholog: 8p23.1Cellular Component: cytoplasm; nucleolus; nucleusMolecular Function: 3'-5' exonuclease activity; ribosome binding; rRNA bindingBiological Process: exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); rRNA 3'-end processing
