The ERCC4 Polyclonal Antibody (CAB8119) is a powerful tool for researchers studying ERCC4, a protein involved in DNA repair processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the ERCC4 protein, this antibody allows for the precise detection and analysis of ERCC4 in various cell types.ERCC4, also known as XPF, plays a crucial role in maintaining genomic stability by participating in the repair of DNA damage. Dysregulation of ERCC4 has been linked to various diseases, including cancer and neurodegenerative disorders.
Therefore, studying the function of ERCC4 is essential for understanding the mechanisms underlying these conditions and developing targeted therapies.With its specificity and reliability, the ERCC4 Polyclonal Antibody is an invaluable tool for researchers in the fields of genomics, oncology, and molecular biology. By enabling the accurate detection and measurement of ERCC4 expression, this antibody facilitates in-depth investigations into the role of ERCC4 in DNA repair pathways and disease pathogenesis.
Product Name:
ERCC4 Rabbit Polyclonal Antibody
SKU:
CAB8119
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 513-782 of human ERCC4 (NP_005227.1).
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
XPF; RAD1; FANCQ; XFEPS; ERCC11; ERCC4
Positive Sample:
U-251MG,HT-1080
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
104kDa
Observed MW:
120kDa
The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).
Purification Method:
Affinity purification
Gene ID:
2072
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using ERCC4 Rabbit pAb (CAB8119) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
