The ERCC2 Polyclonal Antibody (CAB5640) is a powerful tool for researchers studying the ERCC2 gene, which plays a key role in DNA repair and maintenance. This antibody is produced in rabbits and has been shown to be highly specific for detecting ERCC2 protein in human samples, making it ideal for use in Western blot applications.ERCC2 is essential for the repair of DNA damage caused by various environmental factors, such as UV radiation and chemical exposures. Mutations in the ERCC2 gene have been linked to hereditary diseases like xeroderma pigmentosum, which increases the risk of skin cancer due to impaired DNA repair mechanisms.
By targeting ERCC2 with this polyclonal antibody, researchers can better understand the role of this gene in maintaining genomic stability and preventing the accumulation of mutations that can lead to cancer and other diseases. This antibody is a valuable tool for studies in molecular biology, cancer research, and genetic disorders related to DNA repair pathways.
Product Name:
XPD/ERCC2 Rabbit Polyclonal Antibody
SKU:
CAB5640
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human XPD/XPD/ERCC2 (NP_001124339.1).
The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2068
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using XPD/XPD/ERCC2 antibody (CAB5640) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
