The ERCC2 Polyclonal Antibody (CAB14563) is a vital tool for researchers studying the ERCC2 protein, which plays a crucial role in DNA repair and maintenance. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the ERCC2 protein, this antibody allows for accurate detection and analysis in various cell types, making it an essential component in studies related to DNA repair mechanisms, genomic stability, and cancer research.ERCC2, also known as Xeroderma pigmentosum group D protein, is a key player in the nucleotide excision repair pathway, essential for repairing UV-induced DNA damage and maintaining genomic integrity.
Dysregulation of ERCC2 has been linked to increased susceptibility to cancer and other genetic disorders, making it a promising target for therapeutic interventions. Understanding the function and regulation of ERCC2 is crucial for developing novel treatments and strategies for cancer prevention and therapy. Overall, the ERCC2 Polyclonal Antibody (CAB14563) serves as a valuable tool for researchers investigating the role of ERCC2 in DNA repair processes, genetic stability, and disease development. Its high specificity and sensitivity make it an ideal choice for studies aiming to unravel the molecular mechanisms underlying various diseases and conditions related to DNA damage and repair.
Product Name:
XPD/ERCC2 Rabbit Polyclonal Antibody
SKU:
CAB14563
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human XPD/XPD/ERCC2 (NP_001124339.1).
The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2068
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using XPD/ERCC2 antibody (CAB14563) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
