The ERCC1 Polyclonal Antibody (CAB5291) is a valuable tool for researchers studying ERCC1, a key protein involved in DNA repair processes. This antibody, derived from rabbit hosts, shows high specificity and reactivity with ERCC1 in human samples, making it ideal for use in Western blot applications. By binding to the ERCC1 protein, this antibody enables precise detection and analysis in various cell types, providing researchers with a reliable tool for investigating DNA repair mechanisms and potential therapeutic targets.ERCC1, a critical component of the nucleotide excision repair pathway, plays a crucial role in maintaining genomic stability and preventing DNA damage accumulation.
Dysregulation of ERCC1 has been implicated in various diseases, including cancer and neurodegenerative disorders, highlighting its importance in cellular health and disease progression. By studying ERCC1 expression and activity, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially develop targeted therapies for improved patient outcomes.
Product Name:
ERCC1 Rabbit Polyclonal Antibody
SKU:
CAB5291
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human ERCC1 (NP_973730.1).
The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.
Purification Method:
Affinity purification
Gene ID:
2067
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of Rat skeletal muscle, using ERCC1 antibody (CAB5291) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
