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Equine Tumor Necrosis Factor Alpha (TNFa) ELISA Kit

SKU:
HRDL00038
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P29553
Sensitivity:
13.4pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
DIF,TNF-A,TNFSF2,Cachectin,Tumor Necrosis Factor Ligand Superfamily Member 2,TNF,Tumor Necrosis Factor
Reactivity:
Horse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Equine Tumor Necrosis Factor Alpha (TNFa) ELISA Kit

The Equine Tumor Necrosis Factor Alpha (TNFα) ELISA Kit is specifically designed for the accurate and reliable detection of TNFα levels in horse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.TNFα is a key cytokine involved in inflammation and immune responses, playing a crucial role in various physiological and pathological processes.

Elevated levels of TNFα have been implicated in conditions such as cancer, autoimmune diseases, and infectious diseases, making it a valuable biomarker for studying these disorders and potential treatment options.Overall, the Equine TNFα ELISA Kit is a valuable tool for researchers interested in studying the role of TNFα in equine health and disease, offering accurate and reliable measurements to advance their research efforts.

Product Name:Equine Tumor Necrosis Factor Alpha (TNFa) ELISA Kit
Product Code:HRDL00038
Size:96 Assays
Target:TNFa
Synonyms:DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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