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Equine Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

SKU:
HRDL00033
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O19011
Sensitivity:
6.3pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
TGF-B1,CED,DPD1,LAP,Camurati-Engelmann Disease,Latency-associated peptide
Reactivity:
Horse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Equine Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

The Equine Transforming Growth Factor Beta 1 (TGFB1) ELISA Kit offered by Assay Genie is specifically designed for the accurate detection of TGFB1 levels in equine serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring reliable and reproducible results in a variety of research applications.Transforming Growth Factor Beta 1 (TGFB1) is a key signaling protein involved in various biological processes, including cell growth, differentiation, and immune response regulation.

Dysregulation of TGFB1 is associated with numerous diseases, such as cancer, fibrosis, and inflammatory disorders, making it a critical biomarker for studying these conditions and developing potential therapeutic interventions.The Equine TGFB1 ELISA Kit from Assay Genie is a valuable tool for researchers seeking to accurately measure TGFB1 levels in equine samples, allowing for in-depth analysis of TGFB1's role in health and disease in horses.

Product Name:Equine Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit
Product Code:HRDL00033
Size:96 Assays
Target:TGFb1
Synonyms:TGF-B1, CED, DPD1, LAP, Camurati-Engelmann Disease, Latency-associated peptide
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9991
EDTA plasma (n=5)80-9989
Heparin plasma (n=5)82-9990
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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