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Equine Transferrin (TRF) ELISA Kit

SKU:
HRDL00040
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P27425
Sensitivity:
34ng/mL
Range:
78.125-5000ng/mL
ELISA Type:
Sandwich
Synonyms:
TF,Siderophilin,Serotransferrin,Beta-1 metal-binding globulin
Reactivity:
Horse
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Equine Transferrin (TRF) ELISA Kit

The Equine Transferrin (TRF) ELISA Kit is specially designed for the precise measurement of transferrin levels in equine serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring consistent and accurate results, making it suitable for various research purposes.Transferrin is a vital protein responsible for iron binding and transport in the body, playing a vital role in maintaining iron homeostasis. It is essential for various physiological functions and is closely linked to conditions such as anemia, iron deficiency, and inflammatory disorders.

Detection of transferrin levels can provide valuable insights into these conditions, aiding in research and potential therapeutic developments.Get reliable and precise results with the Equine Transferrin (TRF) ELISA Kit, an essential tool for studying iron metabolism and related disorders in equine health.

Product Name:Equine Transferrin (TRF) ELISA Kit
Product Code:HRDL00040
Size:96 Assays
Target:TRF
Synonyms:TF, Siderophilin, Serotransferrin, Beta-1 metal-binding globulin
Detection Method:Sandwich
Range:78.125-5,000ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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