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Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit (HRDL00037)

SKU:
HRDL00037
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9TUL9
Sensitivity:
0.147ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SFD,TIMP Metallopeptidase Inhibitor 3,Metallopeptidase Inhibitor 3,Sorsby Fundus Dystrophy,Pseudoinflammatory
Reactivity:
Horse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749

Description

system_update_altDatasheet

Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

The Equine TIMP3 (Tissue Inhibitors of Metalloproteinase-3) ELISA Kit is a powerful tool for detecting TIMP3 levels in equine tissue samples. TIMP3 is a key regulator of metalloproteinases, enzymes that play a role in tissue remodeling and extracellular matrix degradation. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for your research needs. TIMP3 is important in various biological processes, including wound healing, inflammation, and cancer progression.

By measuring TIMP3 levels in equine tissues, researchers can gain valuable insights into the role of TIMP3 in various physiological and pathological conditions. This kit is essential for studying equine health and disease, and may provide new avenues for therapeutic interventions.

Product Name:Equine Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit
Product Code:HRDL00037
Size:96 Assays
Target:TIMP3
Synonyms:SFD, TIMP Metallopeptidase Inhibitor 3, Metallopeptidase Inhibitor 3, Sorsby Fundus Dystrophy, Pseudoinflammatory
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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