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Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

SKU:
HRDL00035
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O02722
Sensitivity:
25.3pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
EPO,CLGI,EPA,HCI,Erythroid Potentiating Activity,Collagenase Inhibitor,Metalloproteinase inhibitor 1,Fibroblast collagenase inhibitor
Reactivity:
Horse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit

The Equine Tissue Inhibitors of Metalloproteinase-1 (TIMP1) ELISA Kit is a powerful tool for the accurate measurement of TIMP1 levels in equine tissue samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.TIMP1 is a key inhibitor of metalloproteinases, enzymes involved in tissue remodeling and extracellular matrix degradation. Dysregulation of TIMP1 has been linked to various pathological conditions, including cancer, inflammatory diseases, and tissue fibrosis.

Understanding the levels of TIMP1 in equine tissues can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.Overall, the Equine TIMP1 ELISA Kit is a valuable resource for researchers studying equine health and disease, providing a reliable method for investigating the role of TIMP1 in physiological processes and pathological conditions in horses.

Product Name:Equine Tissue Inhibitors Of Metalloproteinase 1 (TIMP1) ELISA Kit
Product Code:HRDL00035
Size:96 Assays
Target:TIMP1
Synonyms:EPO, CLGI, EPA, HCI, Erythroid Potentiating Activity, Collagenase Inhibitor, Metalloproteinase inhibitor 1, Fibroblast collagenase inhibitor
Detection Method:Sandwich
Range:62.5-4,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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