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Equine Matrix Metalloproteinase 7 (MMP7) ELISA Kit

SKU:
HRDL00028
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q4JIK0
Sensitivity:
0.153ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
MPSL1,MAT,PUMP-1,Matrilysin Uterine,Matrilysin,Matrin,Pump-1 Protease,Uterine Metalloproteinase
Reactivity:
Horse
Sample Type:
Serum, Plasma And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Equine Matrix Metalloproteinase 7 (MMP7) ELISA Kit

The Equine Matrix Metalloproteinase 7 (MMP7) ELISA Kit from Assay Genie is a powerful tool for detecting MMP7 levels in equine samples. This kit is specifically designed for use with equine serum, plasma, and cell culture supernatants, offering high sensitivity and specificity for accurate and reliable results.MMP7 is a key enzyme involved in matrix degradation and tissue remodeling, playing a critical role in a variety of physiological and pathological processes. Its dysregulation has been linked to various diseases such as arthritis, cancer, and inflammatory conditions.

The Equine MMP7 ELISA Kit provides researchers with a valuable tool for studying MMP7 function and potential therapeutic interventions.Whether you are studying equine health, disease mechanisms, or drug development, the Equine MMP7 ELISA Kit is an essential kit for your research needs. Trust Assay Genie for high-quality, innovative solutions in the field of equine biomarker research.

Product Name:Equine Matrix Metalloproteinase 7 (MMP7) ELISA Kit
Product Code:HRDL00028
Size:96 Assays
Target:MMP7
Synonyms:MPSL1, MAT, PUMP-1, Matrilysin Uterine, Matrilysin, Matrin, Pump-1 Protease, Uterine Metalloproteinase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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