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Equine Matrix Metalloproteinase 3 (MMP3) ELISA Kit

SKU:
HRDL00027
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q28397
Sensitivity:
1.43ng/mL
Range:
3.125-200ng/mL
ELISA Type:
Sandwich
Synonyms:
SL1,STMY,STMY1,STR1,Progelatinase,Stromelysin 1,Transin-1
Reactivity:
Horse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Equine Matrix Metalloproteinase 3 (MMP3) ELISA Kit

The Equine Matrix Metalloproteinase-3 (MMP-3) ELISA Kit is a valuable tool designed for the accurate measurement of MMP-3 levels in equine serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and dependable results for a variety of research purposes.MMP-3 is a key enzyme involved in the breakdown of extracellular matrix components, playing a crucial role in tissue remodeling and repair processes. Abnormal MMP-3 levels have been linked to various inflammatory and degenerative conditions in horses, making it a valuable biomarker for investigating these diseases and potential therapeutic interventions.

With its user-friendly protocol and high-quality components, the Equine MMP-3 ELISA Kit is an essential tool for researchers and veterinarians studying equine health and disease mechanisms. Trust in this kit to deliver precise and reliable results for your research needs.

Product Name:Equine Matrix Metalloproteinase 3 (MMP3) ELISA Kit
Product Code:HRDL00027
Size:96 Assays
Target:MMP3
Synonyms:SL1, STMY, STMY1, STR1, Progelatinase, Stromelysin 1, Transin-1
Detection Method:Sandwich
Range:3.125-200ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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