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Equine Matrix Metalloproteinase 13 (MMP13) ELISA Kit

SKU:
HRDL00026
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O18927
Sensitivity:
6.9pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CLG3,Collagenase 3
Reactivity:
Horse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Equine Matrix Metalloproteinase 13 (MMP13) ELISA Kit

The Equine Matrix Metalloproteinase-13 (MMP-13) ELISA Kit is a powerful tool for the accurate detection of MMP-13 levels in equine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it perfect for various research applications.MMP-13 is a key enzyme involved in extracellular matrix remodeling and tissue turnover. Its dysregulation has been implicated in various pathological conditions, including arthritis, cancer, and cardiovascular diseases.

Therefore, measuring MMP-13 levels can shed light on underlying disease mechanisms and aid in the development of potential therapeutic interventions.Overall, the Equine MMP-13 ELISA Kit offers researchers a valuable means to investigate the role of MMP-13 in equine health and disease, ultimately contributing to advancements in equine medicine and personalized treatment strategies.

Product Name:Equine Matrix Metalloproteinase 13 (MMP13) ELISA Kit
Product Code:HRDL00026
Size:96 Assays
Target:MMP13
Synonyms:CLG3, Collagenase 3
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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