null

Equine Matrix Metalloproteinase 1 (MMP1) ELISA Kit

SKU:
HRDL00025
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9XSZ5
Sensitivity:
0.040ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CLGN,CLG1,Collagenase,Interstitial Collagenase,Vertebrate Collagenase,Fibroblast Collagenase
Reactivity:
Horse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Equine Matrix Metalloproteinase 1 (MMP1) ELISA Kit

The Equine Matrix Metalloproteinase-1 (MMP1) ELISA Kit is designed for the precise measurement of MMP1 levels in equine serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and consistent results for various research purposes.MMP1 is a key enzyme involved in the breakdown of extracellular matrix proteins, playing a crucial role in tissue remodeling and repair processes. Dysregulation of MMP1 has been linked to various pathological conditions, including arthritis, cardiovascular diseases, and cancer.

Therefore, this kit serves as a valuable tool for studying MMP1 levels in equine samples and gaining insights into related diseases and potential therapeutic interventions.Overall, the Equine MMP1 ELISA Kit offers researchers a reliable and efficient method for evaluating MMP1 expression levels in equine samples, facilitating advances in equine health research and biological studies.

Product Name:Equine Matrix Metalloproteinase 1 (MMP1) ELISA Kit
Product Code:HRDL00025
Size:96 Assays
Target:MMP1
Synonyms:CLGN, CLG1, Collagenase, Interstitial Collagenase, Vertebrate Collagenase, Fibroblast Collagenase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products