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Equine Interleukin 2 (IL-2) ELISA Kit

SKU:
HRDL00018
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P37997
Sensitivity:
10.5pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
TCGF,Lymphokine,T-Cell Growth Factor,Aldesleukin
Reactivity:
Horse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Equine Interleukin 2 (IL-2) ELISA Kit

The Equine Interleukin-2 (IL-2) ELISA Kit is a powerful tool for detecting and quantifying levels of IL-2 in equine samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it invaluable for a wide range of research applications.Interleukin-2 is a key cytokine that plays a critical role in regulating immune responses and inflammation.

By measuring IL-2 levels, researchers can gain valuable insights into the horse's immune system and its response to various challenges, including infections, autoimmune disorders, and cancer. This kit is essential for studying equine immunology and developing new therapies to enhance equine health and well-being.

Product Name:Equine Interleukin 2 (IL-2) ELISA Kit
Product Code:HRDL00018
Size:96 Assays
Target:IL2
Synonyms:TCGF, Lymphokine, T-Cell Growth Factor, Aldesleukin
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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