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Equine Interleukin 12B (IL-12B) ELISA Kit

SKU:
HRDL00011
Product Type:
ELISA Kit
Sensitivity:
12.5pg/mL
Range:
31.2-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
p40,IL12-B,CLMF2,NKSF2,Natural Killer Cell Stimulatory Factor 2,Cytotoxic Lymphocyte Maturation Factor 2,Interleukin 23, P40 Subunit
Reactivity:
Horse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Equine Interleukin 12B (IL-12B) ELISA Kit

The Equine Interleukin-12B (IL12B) ELISA kit from Assay Genie is a cutting-edge tool for detecting interleukin-12B levels in equine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it suitable for a variety of research applications.Interleukin-12B is a key cytokine involved in the immune response, playing a critical role in regulating T cell differentiation and activation.

Dysregulation of IL12B is associated with various inflammatory and autoimmune diseases in horses, making it a valuable biomarker for studying these conditions and developing potential therapies.Trust Assay Genie's Equine Interleukin-12B (IL12B) ELISA kit for precise and reliable measurements of IL12B levels in equine samples, advancing your research and contributing to a better understanding of equine health and disease.

Product Name:Equine Interleukin 12B (IL-12B) ELISA Kit
Product Code:HRDL00011
Size:96 Assays
Target:IL12B
Synonyms:p40, IL12-B, CLMF2, NKSF2, Natural Killer Cell Stimulatory Factor 2, Cytotoxic Lymphocyte Maturation Factor 2, Interleukin 23, P40 Subunit
Detection Method:Sandwich
Range:31.2-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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