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Equine Interleukin 10 (IL-10) ELISA Kit

SKU:
HRDL00010
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q28374
Sensitivity:
5.4pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CSIF,IL10A,TGIF,Cytokine Synthesis Inhibitory Factor
Reactivity:
Horse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Equine Interleukin 10 (IL-10) ELISA Kit

The Equine Interleukin-10 (IL-10) ELISA Kit is a reliable and accurate tool for detecting and measuring interleukin-10 levels in equine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers consistent and reproducible results, making it suitable for a wide range of research applications.Interleukin-10 is a key cytokine that plays a critical role in regulating immune responses and inflammation in horses. It is involved in various physiological and pathological processes, making it a valuable biomarker for studying equine health and disease conditions such as infections, autoimmune disorders, and inflammatory diseases.

Investing in the Equine Interleukin-10 (IL-10) ELISA Kit will provide researchers and veterinarians with a valuable tool to further understand and monitor the immune responses and inflammatory pathways in horses, leading to potential advancements in equine healthcare and therapeutic strategies.

Product Name:Equine Interleukin 10 (IL-10) ELISA Kit
Product Code:HRDL00010
Size:96 Assays
Target:IL10
Synonyms:CSIF, IL10A, TGIF, Cytokine Synthesis Inhibitory Factor
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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