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Equine Alpha-Fetoprotein (aFP) ELISA Kit

SKU:
HRDL00001
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P49066
Sensitivity:
0.116ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
A-FP,FETA,HPAFP,Alpha-Fetoglobulin,Alpha-1-fetoprotein
Reactivity:
Horse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Equine Alpha-Fetoprotein (aFP) ELISA Kit

The Equine Alpha-Fetoprotein (AFP) ELISA Kit is specifically designed for the precise measurement of alpha-fetoprotein levels in equine serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Alpha-fetoprotein is a vital protein that plays a crucial role in fetal development and liver function in horses. Abnormal levels of AFP can indicate certain health conditions such as liver disease, tumors, and reproductive disorders in equines, making it a valuable biomarker for diagnostic and research purposes in equine medicine.

With its high-performance capabilities, the Equine Alpha-Fetoprotein (AFP) ELISA Kit is an essential tool for researchers and veterinarians seeking to study and monitor equine health and disease conditions related to AFP levels.

Product Name:Equine Alpha-Fetoprotein (aFP) ELISA Kit
Product Code:HRDL00001
Size:96 Assays
Target:aFP
Synonyms:A-FP, FETA, HPAFP, Alpha-Fetoglobulin, Alpha-1-fetoprotein
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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