The EPX Polyclonal Antibody (CAB16450) is a valuable tool for researchers studying eosinophil peroxidase (EPX), an enzyme involved in the immune response against parasites and in allergic reactions. Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. By binding specifically to EPX, researchers can detect and analyze its expression in various cell types, providing insights into its role in immunology and inflammatory diseases.
EPX is a key player in the immune response, particularly in the defense against parasites and in allergic reactions. Understanding the function of EPX is essential for developing treatments for parasitic infections, asthma, and other allergic conditions. The EPX Polyclonal Antibody offers a reliable tool for studying this enzyme and its involvement in various immune-related processes.
Product Name:
EPX Rabbit Polyclonal Antibody
SKU:
CAB16450
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EPX (NP_000493.1).
Sequence:
TELR RLNP RWNG DKLY NEAR KIMG AMVQ IITY RDFL PLVL GKAR ARRT LGHY RGYC SNVD PRVA NVFT LAFR FGHT MLQP FMFR LDSQ YRAS APNS HVPL S
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
EPO; EPP; EPXD; EPX-PEN; EPX
Positive Sample:
SW620,human serum
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasmic granule.
Calculated MW:
81kDa
Observed MW:
81kDa
This gene is a member of the peroxidase gene family and is expressed in eosinophils. The encoded preproprotein is proteolytically processed into covalently attached heavy and light chains to form the mature enzyme, which functions as an oxidant. The enzyme is released at sites of parasitic infection or allergen stimulation to mediate lysis of protozoa or parasitic worms. The gene is found in a gene cluster with other peroxidase genes on chromosome 17. Mutations in this gene result in eosinophil peroxidase deficiency.
Purification Method:
Affinity purification
Gene ID:
8288
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using EPX Rabbit pAb (CAB16450) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
