The Eppin Polyclonal Antibody (CAB2149) is a valuable tool for researchers studying Eppin, a protein involved in male fertility and reproductive health. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to Eppin, this antibody enables researchers to detect and analyze the protein in various cell types, making it an essential tool for studies in reproductive biology and male infertility.Eppin is a key player in sperm function and fertility, making it a promising target for research in the fields of reproductive medicine and assisted reproduction.
Understanding the role of Eppin is crucial for developing new treatments for male infertility and improving outcomes for individuals struggling with fertility issues. The Eppin Polyclonal Antibody offers a high level of sensitivity and specificity, making it an indispensable tool for researchers aiming to unravel the complexities of male reproductive health.
Product Name:
EPPIN Rabbit Polyclonal Antibody
SKU:
CAB2149
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 22-133 of human EPPIN (NP_065131.1).
This gene encodes an epididymal protease inhibitor, which contains both kunitz-type and WAP-type four-disulfide core (WFDC) protease inhibitor consensus sequences. Most WFDC genes are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene is a member of the WFDC gene family and belongs to the telomeric cluster. The protein can inhibit human sperm motility and exhibits antimicrobial activity against E. coli, and polymorphisms in this gene are associated with male infertility. Read-through transcription also exists between this gene and the downstream WFDC6 (WAP four-disulfide core domain 6) gene. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
57119
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using EPPIN antibody (CAB2149) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
