The EphB2 Polyclonal Antibody (CAB9813) is a valuable tool for researchers studying EphB2, a receptor tyrosine kinase involved in various cellular processes, including cell adhesion, migration, and axon guidance. This antibody is produced in rabbits and shows high reactivity with human samples, making it suitable for use in Western blot applications. By specifically binding to EphB2, researchers can easily detect and analyze the protein in different cell types, making it ideal for studies in neuroscience, developmental biology, and cancer research.
EphB2 is known to play a crucial role in neuronal development and synaptic plasticity, making it a target of interest for understanding brain function and neurodevelopmental disorders. Its dysregulation has been linked to various neurological conditions, such as Alzheimer's disease and Parkinson's disease, highlighting its potential as a therapeutic target. By studying EphB2 activity, researchers can uncover new insights into neural development and potentially identify novel strategies for treating neurological disorders.
Product Name:
EPHB2 Rabbit Polyclonal Antibody
SKU:
CAB9813
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 250-540 of human EPHB2 (NP_059145.2).
Cell membrane, Cell projection, Single-pass type I membrane protein, axon, dendrite.
Calculated MW:
117kDa
Observed MW:
117kDa
This gene encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins. These receptors are composed of an N-terminal glycosylated ligand-binding domain, a transmembrane region and an intracellular kinase domain. They bind ligands called ephrins and are involved in diverse cellular processes including motility, division, and differentiation. A distinguishing characteristic of Eph-ephrin signaling is that both receptors and ligands are competent to transduce a signaling cascade, resulting in bidirectional signaling. This protein belongs to a subgroup of the Eph receptors called EphB. Proteins of this subgroup are distinguished from other members of the family by sequence homology and preferential binding affinity for membrane-bound ephrin-B ligands. Allelic variants are associated with prostate and brain cancer susceptibility. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2048
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using EPHB2 antibody (CAB9813) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
