The EPHB1 Polyclonal Antibody (CAB8415) is a valuable tool for researchers studying EPHB1, a member of the Eph receptor tyrosine kinase family involved in various cellular processes such as cell adhesion, migration, and axon guidance. This antibody, raised in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the EPHB1 protein, this antibody facilitates the detection and analysis of EPHB1 in different cell types, making it suitable for research in developmental biology, neuroscience, and cancer research.
EPHB1 is known to play a crucial role in neuronal development, including axon guidance and synaptogenesis, as well as in cancer progression and metastasis. Understanding the function and regulation of EPHB1 is essential for unraveling its involvement in these processes and potentially identifying new therapeutic targets for neurological disorders and cancer treatment. The EPHB1 Polyclonal Antibody provides a reliable tool for investigating the role of EPHB1 in various biological contexts, advancing our understanding of its significance in health and disease.
Product Name:
EPHB1 Rabbit Polyclonal Antibody
SKU:
CAB8415
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 260-500 of human EPHB1 (NP_004432.1).
Cell membrane, Cell projection, Early endosome membrane, Single-pass type I membrane protein, dendrite.
Calculated MW:
110kDa
Observed MW:
110kDa
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.
Purification Method:
Affinity purification
Gene ID:
2047
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse kidney, using EPHB1 antibody (CAB8415) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
