The EphA1 Polyclonal Antibody (CAB7328) is optimized for research involving EphA1, a member of the Eph receptor family known for its role in cell signaling and regulation of cell adhesion and migration. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to EphA1 protein, it enables precise detection and analysis in various cell types, making it a valuable tool for studies in developmental biology and cancer research.
EphA1 is a receptor tyrosine kinase involved in diverse biological processes, including cell growth, differentiation, and neuronal development. Its dysregulation has been implicated in various diseases, such as cancer, neurological disorders, and cardiovascular conditions. Investigating the function of EphA1 is essential for understanding its role in these pathologies and developing targeted therapies to modulate its activity for therapeutic purposes.
Product Name:
EPHA1 Rabbit Polyclonal Antibody
SKU:
CAB7328
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human EPHA1 (NP_005223.4).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
108kDa
Observed MW:
100kDa
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis.
Purification Method:
Affinity purification
Gene ID:
2041
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using EPHA1 antibody (CAB7328) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
