The EpCAM Polyclonal Antibody (CAB1177) is a valuable tool for research involving EpCAM, a cell surface glycoprotein that plays a critical role in cell adhesion and signaling. This polyclonal antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in various research applications.EpCAM, also known as epithelial cell adhesion molecule, is commonly overexpressed in cancer cells, making it a promising target for cancer therapy and diagnostics. The EpCAM Polyclonal Antibody enables the detection and analysis of this protein in a wide range of cell types, providing valuable insights into its function and potential roles in cancer progression.
Researchers in the fields of oncology, molecular biology, and cell biology can benefit from using the EpCAM Polyclonal Antibody in techniques such as Western blotting, immunohistochemistry, and flow cytometry. By studying EpCAM expression and localization, researchers can uncover new insights into tumor biology and potentially develop novel strategies for cancer treatment.
Product Name:
EpCAM Rabbit Polyclonal Antibody
SKU:
CAB1177
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 24-265 of human EpCAM (NP_002345.2).
Cell junction, Lateral cell membrane, Single-pass type I membrane protein, tight junction.
Calculated MW:
35kDa
Observed MW:
35-45kDa
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
Purification Method:
Affinity purification
Gene ID:
4072
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using EpCAM antibody (CAB1177) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
