The Envolplakin Polyclonal Antibody (CAB10215) is a valuable tool for researchers studying envoplakin, a key protein involved in the structure and function of epithelial tissues. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in various research applications, including immunohistochemistry and Western blotting.Envoplakin is a crucial component of desmosomes, cell structures that provide strength and stability to epithelial tissues. Dysregulation of envoplakin has been linked to skin disorders and diseases such as pemphigus, making it an important target for investigation in dermatology and skin biology research.
By specifically binding to envoplakin, this antibody enables researchers to detect and analyze the protein in different cell types and tissue samples, providing valuable insights into its role in maintaining tissue integrity and function. Its versatility and reliability make it a valuable tool for studies aiming to understand the molecular mechanisms underlying epithelial tissue biology and pathology.
Product Name:
EVPL Rabbit Polyclonal Antibody
SKU:
CAB10215
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1784-2033 of human EVPL (NP_001979.2).
This gene encodes a member of the plakin family of proteins that forms a component of desmosomes and the epidermal cornified envelope. This gene is located in the tylosis oesophageal cancer locus on chromosome 17q25, and its deletion is associated with both familial and sporadic forms of oesophageal squamous cell carcinoma. Patients suffering from the autoimmune mucocutaneous disorder, paraneoplastic pemphigus, develop antibodies against the encoded protein.
Purification Method:
Affinity purification
Gene ID:
2125
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using EVPL antibody (CAB10215) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
