The ENPP1 Polyclonal Antibody (CAB17876) is designed for research involving ENPP1, an enzyme involved in phosphate metabolism and insulin signaling. The antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications, including Western blot and immunohistochemistry. It specifically binds to the ENPP1 protein, allowing for accurate detection and analysis in a variety of cell types.ENPP1 is known for its role in regulating insulin sensitivity and glucose metabolism, making it a key player in conditions such as diabetes and metabolic syndrome.
Research on ENPP1 is vital for understanding the mechanisms underlying these diseases and developing targeted therapies. With its high specificity and sensitivity, the ENPP1 Polyclonal Antibody is an essential tool for scientists studying metabolic disorders and potential treatment strategies.
Product Name:
Enpp1 Polyclonal Antibody
SKU:
CAB17876
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 580-730 of mouse Enpp1 (NP_032839.3).
This gene encodes a member of the nucleoside pyrophosphatase/phosphodiesterase family of enzymes that catalyzes the hydrolysis of pyrophosphate and phosphodiester bonds in nucleotide triphosphates and oligonucleotides, respectively, to generate nucleoside 5'-monophosphates. The encoded protein is a type II transmembrane glycoprotein that negatively regulates bone mineralization. Mice harboring a nonsense mutation in this gene, termed tiptoe walking (ttw), exhibit ectopic ossification of the spinal ligaments. The encoded protein binds to the insulin receptor, inhibits downstream signaling events and induces insulin resistance and glucose tolerance. This gene is located adjacent to a paralog on chromosome 10. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
18605
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Enpp1 Rabbit pAb (CAB17876) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
