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ENPP1 Antibody (PACO54282)

SKU:
PACO54282
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
IF
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
€339
Frequently bought together:

Description

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ENPP1 Antibody (PACO54282)

The ENPP1 Monoclonal Antibody (PAC054282) is a crucial tool for researchers studying ENPP1, a key enzyme involved in the regulation of pyrophosphate and phosphate levels in the body. This antibody, produced in mice, exhibits high specificity towards human samples and is validated for use in various applications, with a particular focus on immunohistochemistry and ELISA assays.ENPP1, an important regulator of mineralization and calcification processes, is implicated in conditions such as arteriosclerosis and insulin resistance.

By targeting ENPP1, researchers can gain valuable insights into the mechanisms of these diseases and potentially develop new therapeutic strategies. This antibody enables precise detection and quantification of ENPP1 expression in tissues, cells, and biological samples, making it an indispensable tool for studies in the fields of cardiovascular disease and metabolic disorders.

Antibody Name:ENPP1 Antibody (PACO54282)
Antibody SKU:PACO54282
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA, IF
Recommended Dilutions:ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:Human
Immunogen:Recombinant Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 protein (1-75AA)
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:>95%, Protein G purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageImmunofluorescent analysis of HepG2 cells using PACO54282 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:By generating PPi, plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. PPi inhibits mineralization by binding to nascent hydroxyapatite (HA) crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity and function.
Synonyms:Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (E-NPP 1) (Membrane component chromosome 6 surface marker 1) (Phosphodiesterase I/nucleotide pyrophosphatase 1) (Plasma-cell membrane glycoprotein PC-1) [Includes: Alkaline phosphodiesterase I (EC 3.1.4.1); Nucleotide pyrophosphatase (NPPase) (EC 3.6.1.9) (Nucleotide diphosphatase)], ENPP1, M6S1 NPPS PC1 PDNP1
UniProt Protein Function:ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
UniProt Protein Details:

Protein type:Membrane protein, integral; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; EC 3.1.4.1; Phosphatase (non-protein); Motility/polarity/chemotaxis; Carbohydrate Metabolism - starch and sucrose; Cofactor and Vitamin Metabolism - riboflavin; Phosphodiesterase; EC 3.6.1.9

Chromosomal Location of Human Ortholog: 6q22-q23

Cellular Component: extracellular space; cell surface; lysosomal membrane; integral to plasma membrane; basolateral plasma membrane; integral to membrane; plasma membrane

Molecular Function:phosphodiesterase I activity; nucleotide diphosphatase activity; protein homodimerization activity; 3'-phosphoadenosine 5'-phosphosulfate binding; zinc ion binding; calcium ion binding; nucleoside-triphosphate diphosphatase activity; insulin receptor binding; polysaccharide binding; protein binding; nucleic acid binding; scavenger receptor activity; ATP binding

Biological Process: receptor-mediated endocytosis; generation of precursor metabolites and energy; sequestering of triacylglycerol; vitamin metabolic process; nucleoside triphosphate catabolic process; negative regulation of insulin receptor signaling pathway; bone remodeling; negative regulation of fat cell differentiation; phosphate metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process; riboflavin metabolic process; negative regulation of glucose import; cellular phosphate ion homeostasis; cellular response to insulin stimulus; biomineral formation; negative regulation of ossification; immune response; negative regulation of protein amino acid autophosphorylation; negative regulation of cell growth; regulation of bone mineralization; water-soluble vitamin metabolic process; inorganic diphosphate transport; negative regulation of glycogen biosynthetic process

Disease: Obesity; Cole Disease; Arterial Calcification, Generalized, Of Infancy, 1; Hypophosphatemic Rickets, Autosomal Recessive, 2; Diabetes Mellitus, Noninsulin-dependent

NCBI Summary:This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]
UniProt Code:P22413
NCBI GenInfo Identifier:23503088
NCBI Gene ID:5167
NCBI Accession:P22413.2
UniProt Related Accession:P22413
Molecular Weight:
NCBI Full Name:Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
NCBI Synonym Full Names:ectonucleotide pyrophosphatase/phosphodiesterase 1
NCBI Official Symbol:ENPP1  
NCBI Official Synonym Symbols:M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1  
NCBI Protein Information:ectonucleotide pyrophosphatase/phosphodiesterase family member 1
UniProt Protein Name:Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
UniProt Synonym Protein Names:Membrane component chromosome 6 surface marker 1; Phosphodiesterase I/nucleotide pyrophosphatase 1; Plasma-cell membrane glycoprotein PC-1Including the following 2 domains:Alkaline phosphodiesterase I (EC:3.1.4.1); Nucleotide pyrophosphatase (EC:3.6.1.9); NPPase
Protein Family:Ectonucleotide pyrophosphatase/phosphodiesterase family
UniProt Gene Name:ENPP1  
UniProt Entry Name:ENPP1_HUMAN
AntibodiesELISA Kits
ENPP1 Antibody (PACO09096)Human Ectonucleotide pyrophosphatase / phosphodiesterase family member 1 / ENPP1 ELISA Kit
Human Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (ENPP1) ELISA Kit
Secondary Antibody
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)
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